SPECIAL
INSTRUCTIONS: None
Revised 5/19/2010
CC
TEST
NAME: ELECTROLYTE PANEL, Urine, 24
Hours
Synonyms: Lytes
Panel/Profile Components: Sodium (Na+),
Potassium (K+), Chloride
(Cl-)
Patient Preparation: Timed urine
collection
SPECIMEN COLLECTION
Specimen
Type: Urine
Collection Requirement: Collect urine specimen in a
clean container without preservative. Refrigerate specimen during collection
period.
Minimum Volume: 0.5 mL
Cause for Rejection:
Unlabeled specimen or QNS
Specimen
Handling/Stability: Deliver specimen to chemistry for analysis
promptly. If testing will be delayed for more than 2 hours refrigerate
specimen.
Specimen Storage Stability: Samples are maintained
and saved in the laboratory for 7 days at 4
OC.
TEST AVAILABILITY: 7 days/24
hours
TURN AROUND TIME: STAT: 30 Minutes; Routine: 2
Hours
REFERENCE RANGE: Refer
to results report for reference ranges.
SPECIAL INSTRUCTIONS: None
Revised 5/19/2010 CC
TEST NAME: ELECTROPHORESIS
See Specific Type: SERUM PROTEIN, URINE PROTEIN, IMMUNOGLOBULIN
PROFILE
Revised 5/19/2010
CC
TEST NAME: ENCEPHALITIS Ab PANEL, CSF
MML # 91672
Synonyms:
None
Panel/Profile Component: Panel includes HSV
1/2 Ab, ACIF; HSV 1/2 IgM Ab; LCM virus Ab, IFA; Measles (rubeola) G/M Ab, IFA;
Mumps Ab Panel, IFA; Varicella-Zoster, Total/IgM Ab
Patient
Preparation: As for lumbar puncture.
SPECIMEN
COLLECTION
Specimen Type: CSF
Drawing
Requirements: A physician will perform a lumbar puncture according to
standard protocol. Send 3 mL CSF refrigerated in a screw top plastic
vial.
Minimum Volume: 1.0 mL
Cause for
Rejection: Unlabeled specimen, QNS or grossly bloody specimen
Handling/Stability: Specimen is stable for 14 days if
refrigerated; 30 days if frozen and 7 days if at room
temperature.
Specimen Storage Stability: Samples are sent
to Mayo Medical Laboratories for testing.
TEST
AVAILABILITY: Reference laboratory testing performed by Mayo Medical
Laboratories Monday through Friday.
TURN AROUND
TIME: 4-7
days
REFERENCE
RANGE:
HSV 1/2 Ab, ACIF
(CSF) HSV 1/2 IgM Ab, ACIF
(CSF)
Reference Range: HSV 1
<1:8 Reference Range:
<1:1
HSV 2
<1:2 Interpretive
Criteria:
Interpretive
Criteria: <1:1
Antibody not detected
HSV 1 <1:8:
Antibody not detected >/= 1:1
Antibody detected
HSV 2
<1:2:
HSV 1 >/= 1:8 Antibody detected
HSV 2 >/=
1:2
LYMPHOCYTIC
CHORIOMENINGITIS (LCM) VIRUS, Ab, IFC (CSF)
Reference Range: IgG
<1.1
IgM <1.1
Interpretive
Criteria:
<1:1 Antibody not detected
>/= 1:1 Antibody
detected
MEASLES
(RUBEOLA) IgG and IgM Ab PANEL, IFA (CSF) MUMPS Ab PANEL, IFA
(CSF)
Reference Range: IgG
<1:64 Reference Range:
IgG <1:8
IgM
<1:1
IgM <1.1
VARICELLA-ZOSTER VIRUS, Total & IgM Ab,
ACIF/IFA (CSF)
Reference Range: ACIF
<1:2
IFA <1:1
SPECIAL INSTRUCTIONS:
None
Revised 5/19/2010
CC
TEST NAME: ENDOMYSIAL Ab, IgA, Serum
MML #
9360
Synonyms: EA; gluten-sensitive
enteropathy; immunodermatology; malabsorption;
celiac disease
Panel/Profile Component:
None
Patient Preparation:
None
SPECIMEN COLLECTION
Specimen Type: Serum
Drawing
Requirements: Draw blood in a plain red or serum gel tube. Spin down
and send 2 mL serum refrigerated in a screw top plastic vial.
Minimum
Volume: Adutls: 1 mL
Pediatric: 0.5
mL
Cause for Rejection: Unlabeled specimen, QNS, specimen
drawn in wrong tube, significant hemolysis or lipemia.
Specimen
Handling/Stability: Specimen stable to 2 hours at room
temperature
Specimen Storage Stability: Samples are sent to Mayo
Medical Laboratories for testing.
TEST AVAILABILITY: Reference laboratory testing performed by Mayo Medical
Laboratories Monday-Friday 07:00-17:00.
TURN AROUND TIME: 2-7 days
REFERENCE RANGE: Report
includes presence and titer of circulating IgA endomysial antibodies. Negative
in normal individuals; also negative in dermatitis herpetiformis or celiac
disease patients adhering to gluten-free diet.
SPECIAL
INSTRUCTIONS: None
Revised 5/22/2010 CC
TEST NAME: EPSTEIN-BARR VIRUS (EBV) Ab, IgG/IgM and EBNA, Serum
MML #
84421
Synonyms: Anti-EBV serology; infectious mononucleosis
Ab; VCR (viral capsid Ag, IgG/IgM); EBV panel
Panel/Profile
Component: EBV VCA IgG, EBV VCA IgM and EBNA are all analyzed in this
panel
Patient Preparation: None
SPECIMEN
COLLECTION:
Specimen Type: Serum
Drawing Requirements: Draw
blood in a plain red or gold SST tube. Spin down and send 1.0 mL serum
refrigerated in a screw top plastic vial.
Minimum Volume: 0.6
mL
Cause for Rejection: Unlabeled specimen, QNS, specimen
drawn in wrong tube, excessive hemolysis, excessive
icterus.
Specimen Handling/Stability: Specimen stable for 2 hour
at room temperature. Centrifuge and refrigerate within 2 hr.
Specimen Storage Stability: Samples
are sent to Mayo Medical Laboratories for testing.
TEST AVAILABILITY: Reference laboratory testing performed by Mayo Medical
Laboratories Monday-Friday between 09:00 and 18:00 and Sunday at
06:00.
TURN AROUND TIME:1-2 days
REFERENCE RANGE: EBV VCA, IgM:
Negative
EBV VCA,
IgG: Negative
EBNA
Ab: Negative
SPECIAL
INSTRUCTIONS:None
Revised 5/19/2010 CC
TEST NAME: EPSTEIN-BARR VIRUS (EBV) by RAPID PCR, CSF
MML #
81239
Synonyms: infectious
mononucleosis
Panel/Profile Component:
None
Patient Preparation: as for lumbar
puncture
SPECIMEN
COLLECTION:
Specimen Type: CSF
Drawing Requirements: A
physician will perform a lumbar puncture according to standard protocol. Send
0.5 mL CSF refrigerated in a screw top plastic vial. DO NOT CENTRIFUGE
SPECIMEN. The high sensitivity of amplification by PCR requires the specimen to
be processed in an environment in which contamination of the specimen by
Epstein-Barr Virus DNA is not likely.
Minimum Volume: 0.3
mL
Cause for Rejection: Unlabeled
specimen, specimen that have been centrifuges or QNS
Specimen Handling/Stability: Process
specimen promptly and in an environment where contamination of EBV DNA
is unlikely.
Specimen Storage Stability: Samples are sent to Mayo
Medical Laboratories for testing.
TEST AVAILABILITY: Reference laboratory testing performed by Mayo Medical
Laboratories Monday, Wednesday and Friday at 06:00
TURN AROUND TIME: 2-5
days
REFERENCE RANGE: Negative
Positive
results will be reported as EBV DNA DETECTED.
SPECIAL
INSTRUCTIONS:The high sensitivity of amplification by PCR
requires the specimen to be processed in an environment in which contamination
of the specimen by Epstein-Barr Virus DNA is not likely.
Revised
5/22/2010 CC
TEST
NAME: ERYTHROPOIETIN, Serum
MML #
80173
Synonyms: EPO,
EPOGEN
Panel/Profile Component: None
Patient
Preparation: None
SPECIMEN COLLECTION
Specimen
Type: Serum
Drawing Requirements: Draw blood in a plain red of gold SST
tube. Centrifuge and send 0.5 mL serum frozen in a screw top plastic
vial
Minimum Volume: 0.35 mL
Cause for Rejection: Unlabeled specimen, QNS, specimen drawn in
wrong tube, significant hemolysis or lipemia.
Specimen
Handling/Stability: Specimen stable for 2 hours at room
temperature.
Specimen Storage Stability: Samples are sent to Mayo
Medical Laboratories for testing.
TEST AVAILABILITY: Reference laboratory testing performed by Mayo Medical Laboratories
Monday-Friday 05:00-12:00 and Saturday 06:00-18:00.
TURN AROUND TIME: 1-3
days
REFERENCE
RANGE: Males: 4.0-16
mlU/mL
Females: 4.0-21
mlU/mL
SPECIAL
INSTRUCTIONS: Send
specimen frozen to Mayo Medical Laboratories for
testing.
Revised 5/22/2010 CC
TEST
NAME: ESTRADIOL, Serum
Synonyms: Estradiol-17b , Estrogen, Female Hormone
Panel/Profile Component: None
Patient
Preparation: None
SPECIMEN COLLECTION
Specimen
Type: Serum
Drawing Requirements: Draw blood in a Gold SST or plain red
tube.
Minimum Volume: 0.5 mL
Cause for
Rejection: Unlabeled specimen, QNS, or specimen drawn in wrong
tube.
Specimen
Handling/Stability: Allow samples to clot adequately before
centrifugation. Before placing samples on the system, ensure that the samples
are free of fibrin or other particulate matter and free of bubbles.
Specimen Storage
Stability: Specimen stable for 7 days at 2 ? 8 OC.
TEST AVAILABILITY: 7 Days a
week 06:00-13:30
TURN AROUND TIME: Routine tests are verified by 4 hours if during testing hours.
REFERENCE
RANGE: Males: 0.52 pg/mL
Menstruating females (follicular phase, -12 days): 11-69
pg/mL
Menstruating females (follicular phase, -4
days): 63-165 pg/mL
Menstruating females
(midcycle, -1 day): 146-526 pg/mL
Menstruating
females (luteal phase, +2 days): 33-150 pg/mL
Menstruating females (luteal phase, +6 days): 68-196
pg/mL
Menstruating females (luteal phase, +12
days): 36-133 pg/mL
Postmenopausal females (untreated): 0-37
pg/mL
No critical values
established.
SPECIAL INSTRUCTIONS:
None
Revised 5/22/2010
CC
TEST
NAME: ESTRADIOL, Serum
MML #
81816
Synonyms:
None
Panel/Profile Component:
None
Patient Preparation: None
SPECIMEN COLLECTION
Specimen Type:
Serum
Drawing
Requirements: Draw blood in a plain red or gold SST tube. Centrifuge and
send 1.2 mL serum refrigerated in a screw top plastic
vial.
Minimum Volume: 0.6
mL
Cause for Rejection: Unlabeled specimen, QNS, specimen drawn in
wrong tube.
Specimen Handling/Stability: Samples are stable at room
temperature for 2 hours.
Specimen Storage Stability:
Specimen stable for 7 days at 2 ? 8 OC.
TEST AVAILABILITY:
Reference laboratory testing performed by Mayo Medical
Laboratories Monday-Friday at 13:00.
TURN AROUND
TIME: 2-4 days
REFERENCE
RANGE:
CHILDREN*
1-14 days: Estradiol levels in newborns are very elevated at birth but
will ball to prepubertal levels within a few days.
Males
Tanner Stages# Mean
Age Reference
Range
Stage
I 7.1
Undetectable-13 pg/mL
(>14 days and prepubertal)
Stage
II
12.1 Undetectable-16 pg/mL
Stage
III
13.6 Undetectable-26 pg/mL
Stage
IV
15.1 Undetectable-38 pg/mL
Stage
V 18
10-40 pg/mL
#Puberty onset (transition form Tanner stage
I to Tanner stage II) occurs for boys at a median
age of
11.5 (+/- 2) years. For boys, there is no proven relationship between puberty
onset and
body
weight or ethnic origin. Progression through Tanner stages is variable. Tanner
stage V
(adult)
should be reached by age 18.
Females
Tanner Stages# Mean
Age Reference Range
Stage
I 7.1
Undetectable-20 pg/mL
(>14 days and prepubertal)
Stage
II
10.5 Undetectable-24 pg/mL
Stage
III 11.6
Undetectable-60 pg/mL
Stage
IV 12.3 15-85
pg/mL
Stage
V 14.5
15-350 pg/mL**
#Puberty onset (transition form Tanner stage I to
Tanner stage II) occurs for girls at a
median age of 10.5 (+/- 2) years.
There is evidence that it may occur up to 1 year
earlier in obese girls and
in African-American girls. Progression through Tanner stages
is
variable. Tanner stage V (adult) should be reached by age 18.
*The
reference ranges for children are based on published literature (1,2),
cross-correlation of our assay with assays used to generate the literature data
and on our data for young adults.
ADULTS
Males: 10-40 pg/mL
Females:
Premenopausal: 15-350 pg/mL**
Post menopausal: <10 pg/mL
**E2 levels vary widely
through the menstrual cycle.
Conversion
factor
E2: pg/mL x 3.676 = pmol/L (molecular wt = 272)
SPECIAL
INSTRUCTIONS: None
Revised 5/22/2010 CC
TEST
NAME: ESTRONE, Serum
MML #
81418
Synonyms: Estrogen, Female
Hormone
Panel/Profile Component: None
Patient
Preparation: None
SPECIMEN
COLLECTION
Specimen
Type: Serum
Drawing
Requirements: Draw specimen in a plain red or gold SST tube. Centrifuge
within 24 hours of collection and send Collect 1.2 mL serum refrigerated in a
screw top plastic vial.
Minimum Volume: 0.5
mL
Cause for
Rejection: Unlabeled specimen, QNS, specimen drawn in wrong
tube.
Specimen Handling/Stability: Specimen
stable for 24 hours at room temperature. Serum must be separated from gel within 24 hours of
draw.
Specimen Storage
Stability: Specimen sent to Mayo Medical Laboratories for
testing
TEST AVAILABILITY:
Reference laboratory testing performed
by Mayo Medical Laboratories Monday through Friday at
13:00.
TURN AROUND
TIME: 2-4 Days
REFERENCE VALUES:
CHILDREN*
1-14 Days: Esterone levels in newborns are very
elevated at birth but will pall to prepubertal levels within a few
days.
MALES:
Tanner
Stages Mean
Age Reference Range
Stage
I#:
7.1 Undetectable- 16 pg/mL
(>14 days and prepubertal)
Stage
II:
11.5 Undetectable- 22 pg/mL
Stage
III:
13.6 10-25 pg/mL
Stage
IV:
15.1 10-46 pg/mL
Stage V:
18.1 10-60 pg/mL
#Puberty onset (transition from Tanner Stage I to Tanner Stage II)
occurs for boys at median age of 11.5 (+/-2) years. For boys, there is no
proven relationship between puberty onset and body weight or ethnic origin.
Progression through Tanner stages is variable. Tanner stage V (adult) should be
reached by age 18.
FEMALES:
Tanner Stages
Mean Age Reference
Range
Stage
I#:
7.1 Undetectable-29
pg/mL
(>14 days and prepubertal)
Stage
II:
10.5 10-33 pg/mL
Stage
III: 11.6 15-43
pg/mL
Stage
IV: 12.3 16-77
pg/mL
Stage
V:
14.5 17-200 pg/mL
#Puberty onset (transition from Tanner stage I to Tanner stage II)
occurs for girls at a median age of 10.5 (+/-2) years. There is evidence that
it may occur up to 1 year earlier in obese girls and in Aftican=American girls.
Progression through Tanner stages is variable. Tanner stage V (adult) should be
reached by age 18 years.
*The reference ranges for children are based on
the published literature (1,2), cross correlation of our assay with assays used
to generate the literature data and on our data for young
adults.
ADULTS
Males:
10-60 pg/mL
Females: Premenopausal: 17-200
pg/mL
Postmenopausal: 7-40 pg/mL
Conversion factor: +
E1: pg/mL x 3.704 = pmol/L (molecular wt =
270)
SPECIAL INSTRUCTIONS:
Serum must
be separated from gel within 24 hours of draw. Send
refrigerated specimen to Mayo Medical Laboratories for
testing.
Revised 5/22/2010 CC
TEST NAME: ETHANOL
BREATH SCREEN, Breath
Call the laboratory for specific
instructions from testing personnel.
Revised 5/22/2010 CC
TEST
NAME: ETHANOL, Plasma
Synonyms: alcohol, ethyl alcohol, blood alcohol,
ETOH
Panel/Profile Component: None
Patient Preparation:
None
SPECIMEN
COLLECTION
Specimen Type:
Plasma
Drawing
Requirements: DO NOT USE AN ALCOHOL PAD TO
CLEANSE THE SKIN PRIOR TO SPECIMEN COLLECTION. Draw blood
in a gray top sodium flouride tube.
Minimum Volume:
0.5 mL plasma
Cause for
Rejection: Unlabeled specimen, QNS, specimen drawn in wrong tube, not drawn under chain of
custody.
** Specimen must be collected with chain of custody
and no alcohol pad used to cleanse skin. A chain of custody
can be obtained from the laboratory. No ethanol testing will be performed
with out a properly completed chain of custody.
Specimen
Handling/Stability: Specimen stable for 8 hours at room temperature.
Specimen Storage Stability: Samples are maintained and saved in
the laboratory for 6 months at 4 OC.
TEST AVAILABILITY: 7 Days/24
Hours
TURN AROUND TIME: STAT: 45 Minutes Routine: 2
Hours
REFERENCE
RANGES: Legal
Intoxication Critical
Level
LD50
>80 mg/dL
(0.080%) >300
mg/dL >400 mg/dL
SPECIAL INSTRUCTIONS: Specimen must be always be collected under Chain of
Custody.
Revised 5/22/2010 CC
TEST NAME: ETHANOL, Whole Blood
MML #
8264
Synonyms: alcohol, ethyl alcohol,
blood alcohol, ETOH
Panel/Profile Component:
None
Patient Preparation:
None
SPECIMEN
COLLECTION:
Specimen Type: Whole blood
Drawing
Requirements: Draw blood in a grey-top (potassium oxalate/sodium
fluoride) tube and send 2 mL of potassium oxalate/sodium fluoride whole blood.
Specimen must be sent in original vacutainer at ambient temperature but
refrigerated or frozen specimen will also be accepted. For situations were
chain-of-custody (COC) is required, call the laboratory for a Mayo Medical
Laboratories Chain-of-Custody kit.
Minimum Volume: 0.5
mL
Cause for Rejection: Unlabeled specimen, QNS, specimen
drawn in wrong tube
Specimen Handling/Stability: Specimen
stable for 8 hour at room temperature.
Specimen Storage Stability: Specimen stable for 7 days at -20
OC.
TEST
AVAILABILITY: Reference laboratory
testing performed by Mayo Medical Laboratories Monday-Sunday at various
times.
TURN AROUND TIME:1-2 days
REFERENCE
RANGES: Not
detected (positive results are quantified)
Limit of detection: 10
mg/dL (0.01 g/dL)
Legal limit of intoxication is
80 mg/dL (0.08 g/dL)
Toxic concentration is dependent upon
individual usage history
Potentially lethal concentration: >/= 400 mg/dL (0.4
g/dL)
SPECIAL INSTRUCTIONS: Specimen must be sent in original vacutainer at ambient
temperature but refrigerated or frozen specimen will also be accepted. For
situations were chain-of-custody (COC) is required, call the laboratory for a
Mayo Medical Laboratories Chain-of-Custody
kit.
Revised 5/22/2010 CC
TEST NAME: ETHOSUXIMIDE (ZARONTIN),
Serum
MML # 8769
Synonyms: Ethosuximide,
Zarontin, Ethosuximide
Panel/Profile Component:
None
Patient Preparation: None
SPECIMEN
COLLECTION:
Specimen Type:
Serum
Drawing Requirements: Draw blood in a plain red or
gold SST tube. Centrifuge and separate from the cells within 2 hours of
collection. Refrigerate serum and send 1.0 mL in a screw top plastic vial.
Minimum Volume: 0.4 mL
Cause for
Rejection: Unlabeled specimen, QNS, specimen drawn in wrong
tube
Specimen Handling/Stability: Specimen stable for 2
hours at room temperature.
Specimen Storage Stability:
Samples are sent to Mayo Medical Laboratories for
testing.
TEST
AVAILABILITY: Reference laboratory testing performed by Mayo Medical
Laboratories Monday-Sunday at various times.
TURN AROUND TIME: 1-3
days
REFERENCE RANGES: Therapeutic
Concentration Toxic
Concentration
40-100
ug/mL >/= 100 ug/mmoL
SPECIAL
INSTRUCTIONS: Serum must be separated from the red cells within
2 hours of collection. Send specimen refrigerated in a screw top plastic vial.
Revised 5/22/2010 CC
TEST NAME: ETHYLENE GLYCOL
MML #
8749
Synonyms:
None
Panel/Profile Components: None
Patient
Preparation: None
SPECIMEN
COLLECTION
Specimen Type: Serum
Drawing
Requirements: Draw blood in a plain red - NO SERUM GEL TUBE
ACCEPTED. Centrifuge and send 2 mL serum in s screw top plastic vial
at room temperature.
Minimum Volume: 0.3
mL
Cause for Rejection: Unlabeled specimen, QNS, specimen drawn in wrong
tube
Specimen Handling/Stability: Specimen stable for 2
hours at room temperature.
Specimen Storage Stability:
Samples are sent to Mayo Medical Laboratories for
testing.
TEST
AVAILABILITY: Reference laboratory testing performed by Mayo Medical
Laboratories Monday-Sunday at various times.
TURN AROUND TIME: 1-2
days
REFERENCE RANGES: Toxic
Concentration: >/= 20 mmol/L
Lethal Concentration: >20
mmol/L
SPECIAL INSTRUCTIONS: NO SERUM GEL TUBE
ACCEPTED.
Revised 5/23/2010
CC
TEST NAME:
EXTRACTABLE NUCELAR Ag EVALUATION, Serum
MML #
89035
Synonyms: Anti-ENA; Anti-La; Anti-Ro; Anti-Sm (Smith);
Anti-Sjoren's Syndrome; Anti-U1RNP; ENA/DNA; LA; SSA;
SSB
Panel/Profile Component: Tests always performed in this
evaluation include the following: SS-A/Ro Ab, IgG; SS-B/La Ab, IgG; Sm Ab, IgG;
RNP Ab, IgG; Scl 70 Ab, IgG; Jo 1 Ab, IgG
Patient
Preparation: None
SPECIMEN
COLLECTION
Specimen Type:
Serum
Drawing Requirements: Draw blood in a plain
red or gold SST tube. Centrifuge and send 0.5 mL serum refrigerated in a screw
top plastic vial.
Minimum Volume: 0.4 mL
Cause
for Rejection: Unlabeled
specimen, QNS, specimen drawn in wrong tube
Specimen
Handling/Stability: Specimen stable for 2 hours at room temperature.
Specimen Storage Stability: Samples are sent to Mayo
Medical Laboratories for testing.
TEST
AVAILABILITY: Reference laboratory testing performed by Mayo Medical
Laboratories Monday-Saturday at 16:00 and Sunday at 11:00.
TURN AROUND TIME: 1-2
days
REFERENCE RANGES:
SS-A/Ro Ab,
IgG SS-b/La Ab,
IgG Sm Ab, IgG
<1.0 U =
negative <1.0 U =
negative <1.0 U = negative
>/=
1.0 U = positive >/= 1.0 =
positive >/= 1.0 = positive
RNP Ab,
IgG Scl 70 Ab,
IgG Jo 1 Ab, IgG
<1.0
U = negative <1.0 U =
negative <1.0 U =
negative
>/= 1.0 U =
positive >/= 1.0 U =
positive >/= 1.0 U =
positive
SPECIAL INSTRUCTIONS:
None
Revised 5/23/2010 CC