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Chemistry Tests - D & E

Chemistry Tests - D & E
TEST NAME: DEHYDROEPIANDROSTERONE SULFATE, Serum
MML # 8493 

Synonyms: DHEA-S, DHEA-SO(4)
Panel/Profile Component:  None 
Patient Preparation: None

SPECIMEN COLLECTION
Specimen Type: Serum
Drawing Requirements: Draw blood in a gold SST or plain red tube.  Centrifuge and send 0.8 mL serum refrigerated in a screw top plastic vial.
Minimum Volume:  0.25 mL
Cause for Rejection: Unlabeled specimen, QNS, specimen drawn in wrong tube.
Specimen Handling/Stability:  Specimen stable for 2 hours at room temperature.  Process promptly.
Specimen Storage Stability:  Samples are sent to Mayo Medical Laboratories for testing.

TEST AVAILABILITY:   Reference laboratory testing performed by Mayo Medical Laboratories Monday -Friday from 05:00-12:00 and Saturday 0600? 18:00

TURN AROUND TIME:  1-3 days

REFERENCE RANGE:
MALES: 
1-14 Days:  DHEA-S levels in newborns are very elevated at birth but will fall to prepubertal levels within a few days.

*Tanner Stage:  Mean Age        Reference Range (ug/mL)
Stage I              >14 days                 <15-120
Stage II            11.5 years                <15-333
Stage III           13.6 years                <15-312
Stage IV            15.1 years                  29-412
Stage V             18.0 years                  89-457
*Puberty onset (transition from Tanner stage I to Tanner stage II) occurs for boys at median age of 11.5 (+/-) 2 years.  For boys, there is no proven relationship between puberty onse and body weight or ethnic origin.  Progression through Tanner stages is variable.  Tanner stage V (adult) is usually reached by age 18.

18-29 years:  89-457 ug/dL
30-39 years:  65-334 ug/dL
40-49 years:  48-244 ug/dL
50-59 years:  35-179 ug/dL
> or = 60 years:  25-131 ug/dL

FEMALES:
1-14 days:  DHEA-S levels in newborns are very elevated at birth but fall to prepubertal levels within a few days.

*Tanner Stage:  Mean Age        Reference Range (ug/dL)
Stage I             >14 days         16-96
Sage II             10.5 years       22-184
Stage III           11.6 years       <15-296
Stage IV           12.3 years       17-343
Stage V            14.5 years       44-332
*Puberty onset (transition from Tanner stage I to Tanner stage II) occurs for girls at a median age of 10.5 (+/-) 2 years.  There is evidence that it may occur up to 1 year earlier in obese girls and in African-American girls.  Progression through Tanner stages is variable.  Tanner stage V (adult) is usually reached by age 18.

18-29 years:  44-332 ug/dL
30-39 years:  31-228 ug/dL
40-49 years:  18-244 ug/dL
50-59 years:  <15-200 ug/dL
> or = 60 years:  <15-157 ug/dL
 
SPECIAL INSTRUCTIONS:  None

Revised 5/18/2010 CC


TEST NAME: DEPAKENE
(See VALPROIC ACID)
Revised 9/10/2009 cc

 

TEST NAME:  DESIPRAMINE, Serum
MML # 81854
Synonyms:  Norpramin, TCA
Panel/Profile Component:  None
Patient Preparation:  None
SPECIMEN COLLECTION
Specimen Type: Serum 
Drawing Requirements:  Draw blood in a plain red tube immediately before next dose (a minimum of 12 hours after last dose.)  SERUM GEL (SST) TUBES ARE NOT ACCEPTABLE.  Centrifuge within 2 hours and send 3 mL serum refrigerated. 
Minimum Volume:  1.1 mL
Cause for Rejection: Unlabeled specimen, QNS, specimen drawn in wrong tube.
Specimen Handling/Stability:  Draw blood immediately before next dose (a minimum of 12 hours after last dose) and process within 2 hours.  Send specimen refrigerated. 
Specimen Storage Stability:  Samples are sent to Mayo Medical Laboratories for testing.

TEST AVAILABILITY:   Reference laboratory testing performed by Mayo Medical Laboratories Monday-Saturday at various times.

TURN AROUND TIME:  3-6 days

REFERENCE RANGE:  Therapeutic Concentration:  100-300 ng/mL
                                  Critical Concentration:  >/= 500 ng/mL
                                   
SPECIAL INSTRUCTIONS:   Draw blood immediately before next dose (a minimum of 12 hours after last dose) and process within 2 hours.  Send specimen refrigerated. 

 Revised 5/18/2010 CC


TEST NAME:  DHEA (DEHYDROEPIANDROSTERONE), Serum
MML # 81405

Synonyms:  DHEA
Panel/Profile Component:  None
Patient Preparation:  None

SPECIMEN COLLECTION
Specimen Type:  Serum
Drawing Requirements:  Draw blood in a plain red serum tube.  Spin down and send 1 mL serum in a screw top plastic vial.
Minimum Volume:  0.35 mL
Cause for Rejection: Unlabeled specimen, QNS, specimen drawn in wrong tube.
Specimen Handling/Stability:  Specimen stable for 2 hours at room temperature.
Specimen Storage Stability:  Samples are sent to Mayo Medical Laboratories for testing. 

TEST AVAILABILITY:   Reference laboratory testing performed at Mayo Medical Laboratories Monday-Friday at 09:00.

TURN AROUND TIME:  2-6 days

REFERENCE RANGE:  Premature:  <40 ng/mL*                         (NIH Units)
                                    0-1 day:  <11 ng/mL*                             Premature:  <4,000 ng/dL*
                                    2-6 days:  <8.7 ng/mL*                          0-1 day:  <1,1000 ng/dL*
                                    7 days-1 month:  <5.8 ng/mL*               2-6 days:  <870 ng/dL*
                                    >1-23 months:  <2.9 ng/mL*                  7 days-1 month:  <580 ng/dL
                                    2-5 years:  <2.3 ng/mL                          >1 month-23 months:  <290 ng/dL*
                                    6-10 years:  <3.4 ng/mL                        2-5 years:  <230 ng/dL
                                    11-14 years:  <5.0 ng/mL                      6-10 years:  <340 ng/dL
                                    15-18 years:  <6.6 ng/mL                      11-14 years:  <500 ng/dL
                                    19-30 years:  <13 ng/mL                       15-18 years:  <660 ng/dL            
                                    31-40 years:  <10 ng/mL                       19-30 years:  <1,300 ng/dL
                                    41-50 years:  <8.0 ng/mL                       31-40 years:  <1,100 ng/dL
                                    51-60 years:  <6.0 ng/mL                       41-50 years:  <800 ng/dL
                                    >/= 60 years:  <5.0 ng/mL                      51-60 years:  <600 ng/dL
                                                                                                   >/= 60 years:  <500 ng/dL
*Source:  Dehydroepiandrosterone.  In Pediatric Reference Ranges.  5th edition.  Edited by SJ Soldin, C Brugnara, EC Wong.  Washington, DC, AACC Press, 2005, p 75

SPECIAL INSTRUCTIONS:   None

 Revised 5/19/2010 CC


TEST NAME:  DIAZEPAM and NORDIAZEPAM, Serum
MML # 8629
Synonyms:  Valium, Clorazepate, Tranxene
Panel/Profile Component:  None
Patient Preparation:  None
SPECIMEN COLLECTION
Specimen Type: Serum 
Drawing Requirements:  Draw blood in a plain red tube (GEL TUBES NOT ACCEPTABLE.)  Centrifuge and send 3.0 mL serum refrigerated serum in a screw top plastic vial.  
Minimum Volume:  1.1 mL
Cause for Rejection: Unlabeled specimen, QNS, specimen drawn in wrong tube.
Specimen Handling/Stability:  Remove serum from red cells and send 3.0 mL refrigerated to Mayo Medical Laboratories for testing. 
Specimen Storage Stability:  Samples sent to Mayo Medical Laboratories for testing. 

TEST AVAILABILITY:   Reference laboratory testing performed by Mayo Medical Laboratories on various days.

TURN AROUND TIME:  2-9 days

REFERENCE RANGE:  Therapeutic Concentration:  Diazepam:  0.2-0.8 ug/mL
                                                                                        Nordiazepam:  0.2-1.0 ug/mL
                                                            Total for both:  0.4-1.8 ug/dL
                              Toxic Concentrations for both:  >/=5.0 ug/mL

SPECIAL INSTRUCTIONS:
None

 Revised 5/19/2010 CC

 
TEST NAME: DIGITOXIN, Serum or Plasma
MML # 91454

Synonyms:  None
Panel/Profile Component:  None
Patient Preparation:  None

SPECIMEN COLLECTION
Specimen Type: Serum or plasma
Drawing Requirements:  Draw blood in a plain red tube (serum gel tubes not acceptable) a lavender EDTA tube.  Centrifuge and send 1.0 mL serum or plasma refrigerated in a screw top plastic vial.
Minimum Volume:  0.25 mL
Cause for Rejection: Unlabeled specimen, QNS, specimen drawn in wrong tube.
Specimen Handling/Stability:  Remove serum or plasma from red cells and send 1.0 mL refrigerated to Mayo Medical Laboratories for testing. 
Specimen Storage Stability:  Samples are sent to Mayo Medical Laboratories for testing. 

TEST AVAILABILITY:   Reference laboratory testing performed at Mayo Medical Laboratories Monday, Wednesday and Friday.

TURN AROUND TIME:  3-6 days

REFERENCE RANGE:  THERAPEUTIC = 10-30 ng/mL
                                    Reporting Limit = 5.0

SPECIAL INSTRUCTIONS:  None

 Revised 5/19/2010 CC

TEST NAME: DIGOXIN

Synonyms: Lanoxin
Panel/Profile Component:  None
Patient Preparation:  None

SPECIMEN COLLECTION

Specimen Type: Serum or plasma (sodium heparin)
Drawing Requirements:  Draw blood in a plain green (sodium heparin) or plain red tube.  Blood specimens for digoxin levels should be collected between six and eight hours after the last oral dose. 
Minimum Volume:  0.5 mL
Cause for Rejection: Unlabeled specimen, QNS, specimen drawn in wrong tube, excessive hemolysis, excessive icterus or patient being treated with Digibind.
Specimen Handling/Stability:  Complete clot formation should take place before centrifugation.  Serum or plasma should be physically separated from cells as soon as possible with a maximum limit of 2 hours from the tlime of collection.  Specimen should be free of particulate matter.
Specimen Storage Stability:  Samples are maintained and saved in the laboratory for 7 days at 4 OC.

TEST AVAILABILITY:  7 Days/24 Hours

TURN AROUND TIME:  STAT:  60 Minutes; Routine:  2 Hours

REFERENCE RANGES:   0.9-2.0 ng/mL
*NOTE:  Therapeutic digoxin concentrations vary significantly, depending on the individual.  A range of 0.9-2.0 ng/mL includes effective serum concentration for many patients; however, some individuals are best treated at concentrations outside this range.  Concentrations greater than 2.0 ng/mL are often associated with toxic symptoms.
                                        

SPECIAL INSTRUCTIONS:  None

Revised 5/19/2010 cc

TEST NAME: DILANTIN
See PHENYTOIN

Revised 5/19/2010 CC


TEST NAME:  DIHYDORXYVITAMIN D 1, 25, Serum
MML # 8822
Synonyms:  Calcitriol; 1,25-dihydroxyvitamin D3
Panel/Profile Component:  None
Patient Preparation:  4 hour fast
SPECIMEN COLLECTION
Specimen Type: Serum 
Drawing Requirements:  Draw blood in a plain red tube.  Centrifuge and send 1.5 mL serum refrigerated in a screw top plastic vial. 
Minimum Volume:  0.7 mL
Cause for Rejection: Unlabeled specimen, QNS, specimen drawn in wrong tube, excessive hemolysis or excessive icterus.
Specimen Handling/Stability:  Complete clot formation should take place before centrifugation.  Serum should be physically separated from cells as soon as possible with a maximum limit of 2 hours from the time of collection.  Specimen should be free of particulate matter.
Specimen Storage Stability:  Samples are sent to Mayo Medical Laboratories for testing. 

TEST AVAILABILITY:  Reference laboratory testing performed by Mayo Medical Laboratories Monday-Friday at 11:00.

TURN AROUND TIME:  2-4 days

REFERENCE RANGES:   Males:  <16 years:  24-86 pg/mL
                                                  >/= 16 years:  18-64 pg/mL
                                 Females:  <16 years:  24-86 pg/mL
                                                  >/= 16 years:  18-78 pg/mL

SPECIAL INSTRUCTIONS:  Patient should be fasting at least 4 hours for most accurate results.
Revised 5/19/2010 CC
TEST NAME: DISOPRYRAMIDE, Serum
MML  # 8220

Synonyms:  Norpace
Panel/Profile Component:  None
Patient Preparation:  None

SPECIMEN COLLECTION
Specimen Type:  serum
Drawing Requirements:   Draw blood in a plain red tube.  Centrifuge and send 1 mL refrigerated serum in a screw top plastic vial.
Minimum Volume: 0.4
Cause for Rejection: Unlabeled specimen, QNS, specimen drawn in wrong tube, excessive hemolysis or excessive icterus.
Specimen Handling/Stability: Specimen stable for 2 hours at room temperature.  
Specimen Storage Stability: Samples are sent to Mayo Medical Laboratories for testing.

TEST AVAILABILITY:  Reference laboratory testing performed by Mayo Medical Laboratories Monday-Sunday at various times.

TURN AROUND TIME:  1-2 days

REFERENCE RANGES:  Therapeutic concentration:  2.0-5.0 mcg/mL
                                      Toxic Concentrations:>7.0 mcg/mL

SPECIAL INSTRUCTIONS:  None

Revised 9/10/2009 cc


TEST NAME: DNA DOUBLE STRANDED (ds-DNA) Ab, IgM, Serum
MML  # 8178

Synonyms:  SLE (systemic lupus erythematosus)
Panel/Profile Component:  Associated with LUPUS and connective tissue disease cascadepanels.
Patient Preparation: 
None

SPECIMEN COLLECTION
Specimen Type: 
serum
Drawing Requirements:   Draw blood in a plain red tube.  Centrifuge and send 0.5 mL refrigerated serum in a screw top plastic vial.
Minimum Volume: 0.4
Cause for Rejection: Unlabeled specimen, QNS, specimen drawn in wrong tube, excessive hemolysis or excessive icterus.
Specimen Handling/Stability: Specimen stable for 2 hours at room temperature.  
Specimen Storage Stability: Samples are sent to Mayo Medical Laboratories for testing.

TEST AVAILABILITY:  Reference laboratory testing performed by Mayo Medical Laboratories Monday-Saturday at 16:00 and Sunday at 11:00.

TURN AROUND TIME:  1-3 days

REFERENCE RANGES:  <5 IU/mL:  Negative (normal)
                                     5-19 IU/mL:  Indeterminate
                                     >/= 10 IU/mL:  Positive

SPECIAL INSTRUCTIONS:  None

Revised 5/19/2010 CC


TEST NAME:  DRUG MECONIUM, Stool

Synonyms:  New born drug screen
Panel/Profile Components:  Drugs tested for amphetamines, cannabinoids, cocaine and metabolites and opiates
Patient Preparation:  None

SPECIMEN COLLECTION
Specimen Type:  stool/feces
Drawing Requirements:  Infants first bowl movement should be collected by nursery.  AIT specimen chain of custody (COC) should be completed by collecting nurse.  Send specimen at room temperature to AIT Laboratories for testing.   
Minimum Volume:  No minimum volume.
Cause for Rejection:  Unlabeled specimen or missed first bowl movement
Specimen Handling/Stability: Specimen stable for shipment at room temperature but refrigerate specimen upon arrival in the laboratory if it is not packaged for shipment immediately.
Specimen Storage Stability:  Samples are sent to AIT Laboratories for testing.

TEST AVAILABILITY:  Reference laboratory testing performed by AIT Laboratories.

TURN AROUND TIME:  5-10 days

REFERENCE RANGE:     Amphetamines:  Negative
                                      Cannabinoids:  Negative
                                      Cocaine Metabolites:  Negative
                                      Opiates:  Negative

SPECIAL INSTRUCTIONSSend specimen and completed chain of custody (COC) via UPS to AIT Laboratories for testing. 

Revised 5/19/2010 CC


TEST NAME:  DRUG SCREEN, Gastric
Please call Reid Hospital Laboratory for specific information.

Revised 5/19/2010 CC


 TEST NAME:  EBV Ab, IgG/IgM and EBNA, Serum
MML # 84421
See Epstein Barr Virus Ab, IgG/IgM and EBNA, Serum

Revised 5/22/2010 CC


TEST NAME:  EHRLICHIA CHAFFEENSIS (HME) IgG, Serum
MML # 81478

Synonyms:  HME, E chaffeensis, tick borne disease
Panel/Profile Component:  None
Patient Preparation:  None

SPECIMEN COLLECTION:
Specimen Type: Serum
Drawing Requirements:  Draw blood in a plain red or gold SST tube.  Spin down and send 0.5 mL serum refrigerated in a screw top plastic vial.  Process promptly.
Minimum Volume: 0.15 mL
Cause for Rejection: Unlabeled specimen, QNS, specimen drawn in wrong tube, excessive hemolysis, excessive icterus.
Specimen Handling/Stability: Specimen stable for 2 hour at room temperature. Centrifuge and refrigerate within 2 hr.
Specimen Storage Stability:  Samples are sent to Mayo Medical Laboratories for testing.

TEST AVAILABILITY:  Reference laboratory testing performed by Mayo Medical Laboratories Monday-Friday at 09:00.

TURN AROUND TIME:1-3 days

REFERENCE RANGE:    <1:64

SPECIAL INSTRUCTIONS:None

Revised 5/19/2010 CC


TEST NAME: ELECTROLYTE PANEL, Serum or Plasma

Synonyms: Lytes
Panel/Profile Components: Sodium (Na+), Potassium (K+) and Chloride (Cl-),
Patient Preparation: None

SPECIMEN COLLECTION:Specimen Type: Serum or plasma
Drawing Requirements:  Draw blood in light green (lithium heparin PST) tube or plain red tube. 
Minimum Volume: 0.5 mL
Cause for Rejection: Unlabeled specimen, QNS, specimen drawn in wrong tube, excessive hemolysis, excessive icterus.
Specimen Handling/Stability: Specimen stable for 4 hour at room temperature. Centrifuge and refrigerate within 4 hr.
Specimen Storage Stability: Samples are maintained and saved in the laboratory for 7 days at 4 OC.

TEST AVAILABILITY:  7 Days/24 Hours

TURN AROUND TIME:  STAT: 30 Minutes; Routine: 2 Hours

REFERENCE RANGE:        Na+ (mmol/L)        K+ (mmol/L)        Cl- (mmol/L)          
136 ? 145                3.5 ? 5.1                 98-107
Note:  Anion gap (AG) = 5-15 mmol/L

SPECIAL INSTRUCTIONSNone

Revised 5/19/2010 CC


TEST NAME: ELECTROLYTE PANEL with pH 

Synonyms: Lytes
Panel/Profile Components: Sodium (Na+), Potassium (K+), Chloride (Cl-) and pH.
Patient Preparation: None

SPECIMEN COLLECTION

Specimen Type: Serum or plasma
Drawing Requirements:  Draw blood in light green (lithium heparin PST) tube or plain red tube. 
Minimum Volume: 0.5 mL
Cause for Rejection: Unlabeled specimen, QNS, specimen drawn in wrong tube, excessive hemolysis, excessive icterus.
Specimen Handling/Stability: Specimen stable for 4 hour at room temperature. Centrifuge and refrigerate within 4 hr.
Specimen Storage Stability: Samples are maintained and saved in the laboratory for 7 days at 4 OC.

TEST AVAILABILITY:  7 Days/24 Hours

TURN AROUND TIME:  STAT: 30 Minutes; Routine: 2 Hours

REFERENCE RANGE:        Na+ (mmol/L)        K+ (mmol/L)        Cl- (mmol/L)          
136 ? 145                3.5 ? 5.1                 98-107
Note:  Anion gap (AG) = 5-15 mmol/L

SPECIAL INSTRUCTIONSNone

Revised 5/19/2010 CC


TEST NAME: ELECTROLYTE PANEL, Urine, Random

Synonyms: Lytes
Panel/Profile Components: Sodium (Na+), Potassium (K+), Chloride (Cl-).
Patient Preparation: None

SPECIMEN COLLECTION
Specimen Type: Urine
Collection Requirement: Collect a random urine specimen in a clean container without preservative. For best results analyze fresh specimen.
Minimum Volume: 0.5 mL
Cause for Rejection: Unlabeled specimen or QNS
Specimen Handling/Stability:  Deliver specimen to chemistry for analysis promptly.  If testing will be delayed for more than 2 hours refrigerate specimen.
Specimen Storage Stability: Samples are maintained and saved in the laboratory for 7 days at 4 OC.

TEST AVAILABILITY:   7 days/24 hours

TURN AROUND TIME:   STAT: 30 Minutes; Routine: 2 Hours

REFERENCE RANGE:        Na+ (mmol/24h)        K+ (mmol/24h)        Cl- (mmol/24h) 
    40-220                       25-125                      110-250
Note:  Anion gap (AG) = 5-15 mmol/L

SPECIAL INSTRUCTIONS:   None

Revised 5/19/2010 CC


TEST NAME: ELECTROLYTE PANEL, Urine, 24 Hours

Synonyms: Lytes
Panel/Profile Components: Sodium (Na+), Potassium (K+), Chloride (Cl-)
Patient Preparation:  Timed urine collection

SPECIMEN COLLECTION
Specimen Type: Urine
Collection Requirement: Collect urine specimen in a clean container without preservative.  Refrigerate specimen during collection period.
Minimum Volume: 0.5 mL
Cause for Rejection: Unlabeled specimen or QNS
Specimen Handling/Stability:  Deliver specimen to chemistry for analysis promptly.  If testing will be delayed for more than 2 hours refrigerate specimen.
Specimen Storage Stability: Samples are maintained and saved in the laboratory for 7 days at 4 OC.

TEST AVAILABILITY:   7 days/24 hours

TURN AROUND TIME:   STAT: 30 Minutes; Routine: 2 Hours

REFERENCE RANGE:  Refer to results report for reference ranges.

SPECIAL INSTRUCTIONS:   None

Revised 5/19/2010 CC


TEST NAME: ELECTROPHORESIS
See Specific Type: SERUM PROTEIN, URINE PROTEIN, IMMUNOGLOBULIN PROFILE

Revised 5/19/2010 CC

TEST NAME:  ENCEPHALITIS Ab PANEL, CSF
MML # 91672

Synonyms:  None
Panel/Profile Component:  Panel includes HSV 1/2 Ab, ACIF; HSV 1/2 IgM Ab; LCM virus Ab, IFA; Measles (rubeola) G/M Ab, IFA; Mumps Ab Panel, IFA; Varicella-Zoster, Total/IgM Ab
Patient Preparation:  As for lumbar puncture.

SPECIMEN COLLECTION
Specimen Type: CSF
Drawing Requirements:  A physician will perform a lumbar puncture according to standard protocol.  Send 3 mL CSF refrigerated in a screw top plastic vial.
Minimum Volume:  1.0 mL 
Cause for Rejection: Unlabeled specimen, QNS or grossly bloody specimen
Handling/Stability:   Specimen is stable for 14 days if refrigerated; 30 days if frozen and 7 days if at room temperature.
Specimen Storage Stability:  Samples are sent to Mayo Medical Laboratories for testing.

TEST AVAILABILITY:   Reference laboratory testing performed by Mayo Medical Laboratories Monday through Friday.

TURN AROUND TIME:  4-7 days

REFERENCE RANGE: 
HSV 1/2 Ab, ACIF (CSF)                                                     HSV 1/2 IgM Ab, ACIF (CSF)                  
Reference Range:  HSV 1 <1:8                                               Reference Range:  <1:1
                            HSV 2 <1:2                                               Interpretive Criteria:
Interpretive Criteria:                                                                    <1:1 Antibody not detected                               
      HSV 1 <1:8:  Antibody not detected                                         >/= 1:1 Antibody detected
      HSV 2 <1:2:                                                                                
      HSV 1 >/= 1:8 Antibody detected
      HSV 2 >/= 1:2

LYMPHOCYTIC CHORIOMENINGITIS (LCM) VIRUS, Ab, IFC (CSF)
Reference Range:  IgG <1.1
                            IgM <1.1
Interpretive Criteria:
      <1:1 Antibody not detected
      >/= 1:1 Antibody detected

MEASLES (RUBEOLA) IgG and IgM Ab PANEL, IFA (CSF)         MUMPS Ab PANEL, IFA (CSF)  
Reference Range:  IgG <1:64                                                       Reference Range:  IgG <1:8
                            IgM <1:1                                                                                    IgM <1.1

VARICELLA-ZOSTER VIRUS, Total & IgM Ab, ACIF/IFA (CSF)
Reference Range:  ACIF <1:2
                            IFA <1:1

SPECIAL INSTRUCTIONS:   None

Revised 5/19/2010 CC                  


TEST NAME:  ENDOMYSIAL Ab, IgA, Serum
MML # 9360

Synonyms:  EA; gluten-sensitive enteropathy; immunodermatology; malabsorption; celiac disease
Panel/Profile Component:  None
Patient Preparation:  None

SPECIMEN COLLECTION
Specimen Type:  Serum
Drawing Requirements:  Draw blood in a plain red or serum gel tube.  Spin down and send 2 mL serum refrigerated in a screw top plastic vial.
Minimum Volume:  Adutls:  1 mL
                           Pediatric:  0.5 mL 
Cause for Rejection: Unlabeled specimen, QNS, specimen drawn in wrong tube, significant hemolysis or lipemia. 
Specimen Handling/Stability:   Specimen stable to 2 hours at room temperature
Specimen Storage Stability:  Samples are sent to Mayo Medical Laboratories for testing.

TEST AVAILABILITY:   Reference laboratory testing performed by Mayo Medical Laboratories Monday-Friday 07:00-17:00.

TURN AROUND TIME:  2-7 days

REFERENCE RANGE:  Report includes presence and titer of circulating IgA endomysial antibodies.  Negative in normal individuals; also negative in dermatitis herpetiformis or celiac disease patients adhering to gluten-free diet. 

 SPECIAL INSTRUCTIONS:   None

Revised 5/22/2010 CC


 TEST NAME:  EPSTEIN-BARR VIRUS (EBV) Ab, IgG/IgM and EBNA, Serum
MML # 84421

Synonyms:  Anti-EBV serology; infectious mononucleosis Ab; VCR (viral capsid Ag, IgG/IgM); EBV panel
Panel/Profile Component:  EBV VCA IgG, EBV VCA IgM and EBNA are all analyzed in this panel
Patient Preparation:  None

SPECIMEN COLLECTION:
Specimen Type: Serum
Drawing Requirements:  Draw blood in a plain red or gold SST tube.  Spin down and send 1.0 mL serum refrigerated in a screw top plastic vial.
Minimum Volume: 0.6 mL
Cause for Rejection: Unlabeled specimen, QNS, specimen drawn in wrong tube, excessive hemolysis, excessive icterus.
Specimen Handling/Stability: Specimen stable for 2 hour at room temperature. Centrifuge and refrigerate within 2 hr.
Specimen Storage Stability:  Samples are sent to Mayo Medical Laboratories for testing.

TEST AVAILABILITY:  Reference laboratory testing performed by Mayo Medical Laboratories Monday-Friday between 09:00 and 18:00 and Sunday at 06:00.

TURN AROUND TIME:1-2 days

REFERENCE RANGE:    EBV VCA, IgM:  Negative
                                      EBV VCA, IgG:  Negative
                                      EBNA Ab:  Negative

SPECIAL INSTRUCTIONS:None

Revised 5/19/2010 CC


 TEST NAME:  EPSTEIN-BARR VIRUS (EBV) by RAPID PCR, CSF
MML # 81239

Synonyms:  infectious mononucleosis
Panel/Profile Component:  None
Patient Preparation:  as for lumbar puncture

SPECIMEN COLLECTION:
Specimen Type: CSF
Drawing Requirements:  A physician will perform a lumbar puncture according to standard protocol.  Send 0.5 mL CSF refrigerated in a screw top plastic vial.  DO NOT CENTRIFUGE SPECIMEN.  The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by Epstein-Barr Virus DNA is not likely.
Minimum Volume: 0.3 mL
Cause for Rejection: Unlabeled specimen, specimen that have been centrifuges or QNS
Specimen Handling/Stability: Process specimen promptly and in an environment where contamination of EBV DNA is unlikely.  
Specimen Storage Stability:  Samples are sent to Mayo Medical Laboratories for testing.

TEST AVAILABILITY:  Reference laboratory testing performed by Mayo Medical Laboratories Monday, Wednesday and Friday at 06:00

TURN AROUND TIME:  2-5 days

REFERENCE RANGE:   Negative
                                    Positive results will be reported as EBV DNA DETECTED.

SPECIAL INSTRUCTIONS:The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by Epstein-Barr Virus DNA is not likely.

Revised 5/22/2010 CC


TEST NAME: ERYTHROPOIETIN, Serum
MML # 80173

Synonyms: EPO, EPOGEN
Panel/Profile Component:  None
Patient Preparation: None

SPECIMEN COLLECTION
Specimen Type: Serum
Drawing Requirements:  Draw blood in a plain red of gold SST tube.  Centrifuge and send 0.5 mL serum  frozen in a screw top plastic vial
Minimum Volume:  0.35 mL 
Cause for Rejection: Unlabeled specimen, QNS, specimen drawn in wrong tube, significant hemolysis or lipemia. 
Specimen Handling/Stability:  Specimen stable for 2 hours at room temperature.
Specimen Storage Stability:  Samples are sent to Mayo Medical Laboratories for testing. 

TEST AVAILABILITY:   Reference laboratory testing performed by Mayo Medical Laboratories Monday-Friday 05:00-12:00 and Saturday 06:00-18:00.

TURN AROUND TIME:  1-3 days

REFERENCE RANGE:   Males:  4.0-16 mlU/mL
                        Females:  4.0-21 mlU/mL


SPECIAL INSTRUCTIONS:  Send specimen frozen to Mayo Medical Laboratories for testing.  

Revised 5/22/2010 CC


TEST NAME: ESTRADIOL, Serum

Synonyms: Estradiol-17b , Estrogen, Female Hormone
Panel/Profile Component:  None
Patient Preparation: None

SPECIMEN COLLECTION
Specimen Type: Serum
Drawing Requirements: Draw blood in a Gold SST or plain red tube. 
Minimum Volume:  0.5 mL
Cause for Rejection: Unlabeled specimen, QNS, or specimen drawn in wrong tube.
Specimen Handling/Stability:  Allow samples to clot adequately before centrifugation.  Before placing samples on the system, ensure that the samples are free of fibrin or other particulate matter and free of bubbles.   
Specimen Storage Stability: Specimen stable for 7 days at 2 ? 8 OC.

TEST AVAILABILITY:  7 Days a week 06:00-13:30

TURN AROUND TIME:  Routine tests are verified by 4 hours if during testing hours. 

REFERENCE RANGE:   Males:  0.52 pg/mL
                            Menstruating females (follicular phase, -12 days):  11-69 pg/mL
                            Menstruating females (follicular phase, -4 days):  63-165 pg/mL
                            Menstruating females (midcycle, -1 day):  146-526 pg/mL
                            Menstruating females (luteal phase, +2 days):  33-150 pg/mL
                            Menstruating females (luteal phase, +6 days):  68-196 pg/mL
                            Menstruating females (luteal phase, +12 days):  36-133 pg/mL
                            Postmenopausal females (untreated):  0-37 pg/mL
                            No critical values established.
SPECIAL INSTRUCTIONS:  None

Revised 5/22/2010 CC

TEST NAME: ESTRADIOL, Serum
MML # 81816

Synonyms:  None
Panel/Profile Component:  None
Patient Preparation: None

SPECIMEN COLLECTION

Specimen Type:  Serum
Drawing Requirements:  Draw blood in a plain red or gold SST tube.  Centrifuge and send 1.2 mL serum refrigerated in a screw top plastic vial.
Minimum Volume:  0.6 mL 
Cause for Rejection: Unlabeled specimen, QNS, specimen drawn in wrong tube.
Specimen Handling/Stability:  Samples are stable at room temperature for 2 hours.
Specimen Storage Stability: Specimen stable for 7 days at 2 ? 8 OC.

TEST AVAILABILITYReference laboratory testing performed by Mayo Medical Laboratories Monday-Friday at 13:00.

TURN AROUND TIME:  2-4 days

REFERENCE RANGE: 
CHILDREN*
      1-14 days:
  Estradiol levels in newborns are very elevated at birth but will ball to prepubertal levels within a few days.

Males
Tanner Stages#                       Mean Age                                Reference Range
Stage I                                           7.1                                    Undetectable-13 pg/mL
     (>14 days and prepubertal)
Stage II                                        12.1                                    Undetectable-16 pg/mL
Stage III                                       13.6                                    Undetectable-26 pg/mL
Stage IV                                        15.1                                   Undetectable-38 pg/mL
Stage V                                         18                                      10-40 pg/mL                
#Puberty onset (transition form Tanner stage I to Tanner stage II) occurs for boys at a median
age of 11.5 (+/- 2) years.  For boys, there is no proven relationship between puberty onset and
body weight or ethnic origin.  Progression through Tanner stages is variable.  Tanner stage V
(adult) should be reached by age 18.

Females
Tanner Stages#                       Mean Age                               
Reference Range
Stage I                                           7.1                                    Undetectable-20 pg/mL
     (>14 days and prepubertal)
Stage II                                        10.5                                    Undetectable-24 pg/mL
Stage III                                       11.6                                    Undetectable-60 pg/mL
Stage IV                                       12.3                                           15-85 pg/mL
Stage V                                        14.5                                           15-350 pg/mL**    
#Puberty onset (transition form Tanner stage I to Tanner stage II) occurs for girls at a
median age of 10.5 (+/- 2) years.  There is evidence that it may occur up to 1 year
earlier in obese girls and in African-American girls.  Progression through Tanner stages
is variable.  Tanner stage V (adult) should be reached by age 18.
*The reference ranges for children are based on published literature (1,2), cross-correlation of our assay with assays used to generate the literature data and on our data for young adults.
ADULTS
Males: 
10-40 pg/mL
Females: 
      Premenopausal:  15-350 pg/mL**
      Post menopausal:  <10 pg/mL

**E2 levels vary widely through the menstrual cycle.
Conversion factor
E2:  pg/mL x 3.676 = pmol/L (molecular wt = 272)

SPECIAL INSTRUCTIONS:  None
Revised 5/22/2010 CC

TEST NAME: ESTRONE, Serum 
MML # 81418

Synonyms: Estrogen, Female Hormone
Panel/Profile Component:  None 
Patient Preparation: None

SPECIMEN COLLECTION

Specimen Type: Serum
Drawing Requirements: Draw specimen in a plain red or gold SST tube.  Centrifuge within 24 hours of collection and send Collect 1.2 mL serum refrigerated in a screw top plastic vial.
Minimum Volume:  0.5 mL
Cause for Rejection: Unlabeled specimen, QNS, specimen drawn in wrong tube.
Specimen Handling/Stability: Specimen stable for 24 hours at room temperature.  Serum must be separated from gel within 24 hours of draw.
Specimen Storage Stability: Specimen sent to Mayo Medical Laboratories for testing

TEST AVAILABILITY:  Reference laboratory testing performed by Mayo Medical Laboratories Monday through Friday at 13:00.

TURN AROUND TIME:  2-4 Days

REFERENCE VALUES: 
CHILDREN* 
1-14 Days:          Esterone levels in newborns are very elevated at birth but will pall to prepubertal levels within a few days.
MALES: 
Tanner Stages                                     Mean Age                                Reference Range
Stage I#:                                                      7.1                                         Undetectable- 16 pg/mL
    (>14 days and prepubertal)
Stage II:                                                     11.5                                         Undetectable- 22 pg/mL
Stage III:                                                    13.6                                         10-25 pg/mL
Stage IV:                                                   15.1                                         10-46 pg/mL
Stage V:                                                    18.1                                         10-60 pg/mL                 
#Puberty onset (transition from Tanner Stage I to Tanner Stage II) occurs for boys at median age of 11.5 (+/-2) years.  For boys, there is no proven relationship between puberty onset and body weight or ethnic origin.  Progression through Tanner stages is variable.  Tanner stage V (adult) should be reached by age 18.

FEMALES:
Tanner Stages                                  Mean Age                                   Reference Range
Stage I#:                                                    7.1                                           Undetectable-29 pg/mL
       (>14 days and prepubertal)
Stage II:                                                    10.5                                          10-33 pg/mL
Stage III:                                                   11.6                                          15-43 pg/mL
Stage IV:                                                  12.3                                          16-77 pg/mL
Stage V:                                                   14.5                                         17-200 pg/mL              
#Puberty onset (transition from Tanner stage I to Tanner stage II) occurs for girls at a median age of 10.5 (+/-2) years.  There is evidence that it may occur up to 1 year earlier in obese girls and in Aftican=American girls.  Progression through Tanner stages is variable.  Tanner stage V (adult) should be reached by age 18 years.

*The reference ranges for children are based on the published literature (1,2), cross correlation of our assay with assays used to generate the literature data and on our data for young adults.

ADULTS
      
Males:  10-60 pg/mL
      Females:  Premenopausal:  17-200 pg/mL
                      Postmenopausal:  7-40 pg/mL

Conversion factor:  +
E1:  pg/mL x 3.704 = pmol/L (molecular wt = 270)

SPECIAL INSTRUCTIONS:   Serum must be separated from gel within 24 hours of draw.  Send refrigerated specimen to Mayo Medical Laboratories for testing.

Revised 5/22/2010 CC

TEST NAME:  ETHANOL BREATH SCREEN, Breath
Call the laboratory for specific instructions from testing personnel.

Revised 5/22/2010 CC


TEST NAME: ETHANOL, Plasma

Synonyms: alcohol, ethyl alcohol, blood alcohol,  ETOH
Panel/Profile Component:  None
Patient Preparation: None

SPECIMEN COLLECTION
Specimen Type:   Plasma
Drawing Requirements DO NOT USE AN ALCOHOL PAD TO CLEANSE THE SKIN PRIOR TO SPECIMEN COLLECTION.  Draw blood in a gray top sodium flouride tube.   
Minimum Volume:  0.5 mL plasma
Cause for Rejection: Unlabeled specimen, QNS, specimen drawn in wrong tube, not drawn under chain of custody.
       ** Specimen must be collected with chain of custody and no alcohol pad used to cleanse skin.  A chain of custody can be obtained from the    laboratory.  No ethanol testing will be performed with out a properly completed chain of custody. 
Specimen Handling/Stability: Specimen stable for 8 hours at room temperature.
Specimen Storage Stability: Samples are maintained and saved in the laboratory for 6 months at 4 OC.

TEST AVAILABILITY:  7 Days/24 Hours

TURN AROUND TIME:  STAT: 45 Minutes Routine: 2 Hours

REFERENCE RANGES:       Legal Intoxication          Critical Level               LD50 
>80 mg/dL (0.080%)          >300 mg/dL           >400 mg/dL

SPECIAL INSTRUCTIONS: Specimen must be always be collected under Chain of Custody. 

Revised 5/22/2010 CC


TEST NAME: ETHANOL, Whole Blood
MML # 8264


Synonyms: alcohol, ethyl alcohol, blood alcohol, ETOH
Panel/Profile Component:  None
Patient Preparation:  None

SPECIMEN COLLECTION:
Specimen Type:  Whole blood
Drawing Requirements:  Draw blood in a grey-top (potassium oxalate/sodium fluoride) tube and send 2 mL of potassium oxalate/sodium fluoride whole blood.  Specimen must be sent in original vacutainer at ambient temperature but refrigerated or frozen specimen will also be accepted.  For situations were chain-of-custody (COC) is required, call the laboratory for a Mayo Medical Laboratories Chain-of-Custody kit. 
Minimum Volume: 0.5 mL
Cause for Rejection: Unlabeled specimen, QNS, specimen drawn in wrong tube
Specimen Handling/Stability: Specimen stable for 8 hour at room temperature.
Specimen Storage Stability: Specimen stable for 7 days at -20 OC.

TEST AVAILABILITY: 
Reference laboratory testing performed by Mayo Medical Laboratories Monday-Sunday at various times.

TURN AROUND TIME:1-2 days

REFERENCE RANGES:      Not detected (positive results are quantified)
Limit of detection:  10 mg/dL (0.01 g/dL)
Legal limit of intoxication is 80 mg/dL (0.08 g/dL)
Toxic concentration is dependent upon individual usage history
Potentially lethal concentration:  >/= 400 mg/dL (0.4 g/dL)

SPECIAL INSTRUCTIONS:  Specimen must be sent in original vacutainer at ambient temperature but refrigerated or frozen specimen will also be accepted.  For situations were chain-of-custody (COC) is required, call the laboratory for a Mayo Medical Laboratories Chain-of-Custody kit.  
Revised 5/22/2010 CC

TEST NAME: ETHOSUXIMIDE (ZARONTIN), Serum
MML # 8769

Synonyms:  Ethosuximide, Zarontin, Ethosuximide
Panel/Profile Component:  None
Patient Preparation:  None

SPECIMEN COLLECTION:
Specimen Type:  Serum
Drawing Requirements:  Draw blood in a plain red or gold SST tube.  Centrifuge and separate from the cells within 2 hours of collection.  Refrigerate serum and send 1.0 mL in a screw top plastic vial. 
Minimum Volume:  0.4 mL
Cause for Rejection: Unlabeled specimen, QNS, specimen drawn in wrong tube
Specimen Handling/Stability: Specimen stable for 2 hours at room temperature.
Specimen Storage Stability:  Samples are sent to Mayo Medical Laboratories for testing. 

TEST AVAILABILITY:  Reference laboratory testing performed by Mayo Medical Laboratories Monday-Sunday at various times.

TURN AROUND TIME:  1-3 days

REFERENCE RANGES:        Therapeutic Concentration               Toxic Concentration
                                                       
40-100 ug/mL                                  >/= 100 ug/mmoL

SPECIAL INSTRUCTIONS:  Serum must be separated from the red cells within 2 hours of collection.  Send specimen refrigerated in a screw top plastic vial. 

Revised 5/22/2010 CC


TEST NAME: ETHYLENE GLYCOL
MML # 8749

Synonyms:  None
Panel/Profile Components:  None
Patient Preparation:  None

SPECIMEN COLLECTION
Specimen Type:  Serum
Drawing Requirements:  Draw blood in a plain red - NO SERUM GEL TUBE ACCEPTED.  Centrifuge and send 2 mL serum in s screw top plastic vial at room temperature.
Minimum Volume:  0.3 mL
Cause for Rejection:  Unlabeled specimen, QNS, specimen drawn in wrong tube
Specimen Handling/Stability: Specimen stable for 2 hours at room temperature.
Specimen Storage Stability:  Samples are sent to Mayo Medical Laboratories for testing. 

TEST AVAILABILITY:  Reference laboratory testing performed by Mayo Medical Laboratories Monday-Sunday at various times.

TURN AROUND TIME:  1-2 days

REFERENCE RANGES:    Toxic Concentration:  >/= 20 mmol/L
                                       Lethal Concentration:  >20 mmol/L

SPECIAL INSTRUCTIONS:  NO SERUM GEL TUBE ACCEPTED.

Revised 5/23/2010 CC

TEST NAME:  EXTRACTABLE NUCELAR Ag EVALUATION, Serum
MML # 89035

Synonyms:  Anti-ENA; Anti-La; Anti-Ro; Anti-Sm (Smith); Anti-Sjoren's Syndrome; Anti-U1RNP; ENA/DNA; LA; SSA; SSB
Panel/Profile Component:  Tests always performed in this evaluation include the following:  SS-A/Ro Ab, IgG; SS-B/La Ab, IgG; Sm Ab, IgG; RNP Ab, IgG; Scl 70 Ab, IgG; Jo 1 Ab, IgG
Patient Preparation:  None

SPECIMEN COLLECTION
Specimen Type:  Serum
Drawing Requirements:  Draw blood in a plain red or gold SST tube.  Centrifuge and send 0.5 mL serum refrigerated in a screw top plastic vial.
Minimum Volume:  0.4 mL
Cause for Rejection:  Unlabeled specimen, QNS, specimen drawn in wrong tube
Specimen Handling/Stability: Specimen stable for 2 hours at room temperature.
Specimen Storage Stability:  Samples are sent to Mayo Medical Laboratories for testing. 

TEST AVAILABILITY:  Reference laboratory testing performed by Mayo Medical Laboratories Monday-Saturday at 16:00 and Sunday at 11:00.

TURN AROUND TIME:  1-2 days

REFERENCE RANGES:  
SS-A/Ro Ab, IgG                                  SS-b/La Ab, IgG                                   Sm Ab, IgG
    
<1.0 U = negative                                  <1.0 U = negative                                   <1.0 U = negative
     >/= 1.0 U = positive                               >/= 1.0 = positive                                    >/= 1.0 = positive
 
RNP Ab, IgG                                        Scl 70 Ab, IgG                                       Jo 1 Ab, IgG
     <1.0 U = negative                                  <1.0 U = negative                                      <1.0 U = negative                     
     >/= 1.0 U = positive                               >/= 1.0 U = positive                                   >/= 1.0 U = positive

SPECIAL INSTRUCTIONS:  None

Revised 5/23/2010 CC



 

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